P-070. Correlation between semen parameters and maturity of normal human spermatozoa as assessed by Acridine Orange staining
نویسندگان
چکیده
منابع مشابه
Correlation between antisperm antibody and Semen parameters
Introduction: The precise mechanism for antisperm antibody (ASA) mediated fertility impairment is unclear. In the male reproductive tract, ASA may have an adverse impact on sperm maturation and function or overall semen quality. In this study, the percentage of ASA-IgA was determined by the direct Mixed Agglutination Reaction (MAR) test in men from infertile couples in Khorramabad city. Further...
متن کاملThe effect of varicocele on sperm morphology and DNA maturity: does acridine orange staining facilitate diagnosis?
OBJECTIVE The aim of this study was to determine whether Acridine Orange (AO) can be used as a routine procedure in a physician's office to detect sperm deoxyribonucleic acid (DNA) fragmentation in patients with varicoceles. MATERIAL AND METHODS Forty-five patients with a mean age of 30.4 years, who had been diagnosed with varicoceles by physical examination and 30 healthy males with a mean a...
متن کاملHuman sperm bound to the zona pellucida have normal nuclear chromatin as assessed by acridine orange fluorescence.
BACKGROUND The aim of this study was to determine if only sperm with double stranded DNA could bind to the human zona pellucida (ZP). METHODS Sperm samples from 124 infertile men with a range of semen abnormalities were studied. Oocytes that had failed to fertilize in IVF or ICSI were used for the sperm-ZP binding test. A group of four oocytes were incubated for 2 h with 2 x 10(6)/ml motile s...
متن کاملAcridine orange staining for rapid diagnosis of Acanthamoeba keratitis.
Acanthamoeba keratitis is uncommon, but one of the most severe infectious diseases of the cornea. Delayed diagnosis or misdiagnosis as bacterial or herpes simplex keratitis leads to extensive corneal inflammation and profound visual loss. Therefore, accurate and rapid diagnosis of Acanthamoeba keratitis is essential for successful treatment and good prognosis. We evaluated the usefulness of acr...
متن کاملHistochemical observations on Mycoplasma after staining with acridine orange.
Mycoplasma colonies and Mycoplasma cells in preparations from infected milk and lymph nodes were observed for their fluorescent qualities after treatment with acridine orange. Mycoplasma colonies fluoresced brilliant red or red-orange. When treated after exposure to ribonuclease, the colonies fluoresced lime-green. There was no fluorescence when both ribonucleic acid and deoxyribonucleic acid w...
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ژورنال
عنوان ژورنال: Human Reproduction
سال: 1997
ISSN: 1460-2350,0268-1161
DOI: 10.1093/humrep/12.suppl_2.153